esoilБюллетень Почвенного института имени В.В. ДокучаеваDokuchaev Soil Bulletin0136-16942312-4202V.V. Dokuchaev Soil Science Institute10.19047/0136-1694-2018-93-105-120esoil-290Research ArticleСтатьиВЫБОР ПОДХОДА ПРИ ИДЕНТИФИКАЦИИ ПОЧВЕННЫХ КОККОИДНЫХ ЗЕЛЕНЫХ МИКРОВОДОРОСЛЕЙ (TREBOUXIOPHYCEAE, CHLOROPHYTA)CHOOSING AN APPROACH FOR IDENTIFICATION OF SOIL COCCOID GREEN MICROALGAE (TREBOUXIOPHYCEAE, CHLOROPHYTA)https://orcid.org/0000-0002-3445-0507ТемралееваА. Д.TemraleevaA. D.

Россия, 142290 Пущино, ул. Институтская, 2* http://orcid.org/0000-0002-3445-0507 е-mail: temraleeva.anna@gmail.com

Russia, 142290, Pushchino, Institutskaya str., 2http://orcid.org/0000-0002-3445-0507 е-mail: temraleeva.anna@gmail.com

temraleeva.anna@gmail.comМоскаленкоС. В.MoskalenkoS. V.

Россия, 142290 Пущино, ул. Институтская, 2

Russia, 142290, Pushchino, Institutskaya str., 2

ПортнаяЕ. А.PortnayaE. A.

Россия, 142290 Пущино, ул. Институтская, 2

Russia, 142290, Pushchino, Institutskaya str., 2

Институт физико-химических и биологических проблем почвоведения РАНРоссияInstitute of physico-chemical and biological problems in soil science RASRussian Federation201801072018093105120Copyright © Темралеева А.Д., Москаленко С.В., Портная Е.А., 20182018Темралеева А.Д., Москаленко С.В., Портная Е.А.Temraleeva A.D., Moskalenko S.V., Portnaya E.A.Данная работа распространяется под лицензией Creative Commons Attribution 4.0.This work is licensed under a Creative Commons Attribution 4.0 License.https://bulletin.esoil.ru/jour/article/view/290

Описываются морфологический и молекулярно-генетический подходы, использованные при идентификации зеленых микроводорослей класса Trebouxiophyceae из коллекции ACSSI. В бурой полупустынной и каштановой почвах были обнаружены водоросли Muriella terrestris, в погребенной лугово-каштановой почве – Edaphochlorella mirabilis. Еще 3 штамма являются новыми неописанными таксонами: ACSSI 014 – новый вид рода Watanabea, изолированный из серой лесной почвы и имеющий пиреноид с крахмальной обверткой в отличии от типового вида, и ACSSI 104 и 144 – Nannochloris-подобный род, представители которого найдены в солонце и каштановой почве и обладают скудными морфологическими признаками. Показано, что ни один критерий в отдельности: морфологический или иной признак, вычисление генетических дистанций, анализ и сравнение вторичной структуры ITS2, поиск компенсаторных замен и молекулярных подписей – не позволяет надежно классифицировать таксоны. В связи с чем необходимо использовать полифазный подход в систематике водорослей, особенно при идентификации таксонов с простой клеточной морфо-логией, которые часто встречаются в почвах.

The article describes the morphological and molecular genetic approaches used to identify the green microalgae of the Trebouxiophyceae class from the ACSSI collection. In the brown semi-desert and chestnut soils, we found Muriella ter-restris. In the buried meadow-chestnut soil we found Edaphochlorella mirabilis. Three more strains are characterized as new uncharacterized taxa: ACSSI 014 is a new species related to the genus Watanabea isolated from gray forest soil, with a pyrenoid surrounded by several starch grains, in contrast to the type species, and ACSSI 104 and 144 are a Nannochloris-like genus, whose representatives were found in solonetz and chestnut soil and are characterized by poor morphological specificities. It is shown that no single criterion (either morphological or other specificity, calculation of genetic distances, analysis and comparison of the ITS2 secondary structure, search for compensatory changes and molecular signatures) does not allow reliable classification of taxa. Therefore, due to this fact, it is necessary to apply a polyphasic approach in the systematics of algae, especially when identifying taxa with simple cellular morphology, which are often found in soils.

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The authors declare that there are no conflicts of interest present.