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<article article-type="research-article" dtd-version="1.3" xmlns:mml="http://www.w3.org/1998/Math/MathML" xmlns:xlink="http://www.w3.org/1999/xlink" xmlns:xsi="http://www.w3.org/2001/XMLSchema-instance" xml:lang="ru"><front><journal-meta><journal-id journal-id-type="publisher-id">esoil</journal-id><journal-title-group><journal-title xml:lang="ru">Бюллетень Почвенного института имени В.В. Докучаева</journal-title><trans-title-group xml:lang="en"><trans-title>Dokuchaev Soil Bulletin</trans-title></trans-title-group></journal-title-group><issn pub-type="ppub">0136-1694</issn><issn pub-type="epub">2312-4202</issn><publisher><publisher-name>V.V. Dokuchaev Soil Science Institute</publisher-name></publisher></journal-meta><article-meta><article-id pub-id-type="doi">10.19047/0136-1694-2020-103-85-107</article-id><article-id custom-type="elpub" pub-id-type="custom">esoil-577</article-id><article-categories><subj-group subj-group-type="heading"><subject>Research Article</subject></subj-group><subj-group subj-group-type="section-heading" xml:lang="ru"><subject>Статьи</subject></subj-group></article-categories><title-group><article-title>Выход денсиметрических фракций из типичных черноземов разного землепользования</article-title><trans-title-group xml:lang="en"><trans-title>The yield values of densimetric fractions from typical chernozems of different land use types</trans-title></trans-title-group></title-group><contrib-group><contrib contrib-type="author" corresp="yes"><contrib-id contrib-id-type="orcid">https://orcid.org/0000-0002-0210-380X</contrib-id><name-alternatives><name name-style="eastern" xml:lang="ru"><surname>Фарходов</surname><given-names>Ю. Р.</given-names></name><name name-style="western" xml:lang="en"><surname>Farkhodov</surname><given-names>Yu. R.</given-names></name></name-alternatives><bio xml:lang="ru"><p>м.н.с. Почвенного института имени В.В. Докучаева, аспирант кафедры земледелия и защиты растений МГУ им. М.В. Ломоносова </p><p>119017, Москва, Пыжевский пер, 7, стр. 2</p></bio><bio xml:lang="en"><p>7 Bld. 2 Pyzhevskiy per., Moscow 119017</p></bio><email xlink:type="simple">yulian.farkhodov@yandex.ru</email><xref ref-type="aff" rid="aff-1"/></contrib><contrib contrib-type="author" corresp="yes"><name-alternatives><name name-style="eastern" xml:lang="ru"><surname>Ярославцева</surname><given-names>Н. В.</given-names></name><name name-style="western" xml:lang="en"><surname>Yaroslavtseva</surname><given-names>N. V.</given-names></name></name-alternatives><bio xml:lang="ru"><p>119017, Москва, Пыжевский пер, 7, стр. 2</p></bio><bio xml:lang="en"><p>7 Bld. 2 Pyzhevskiy per., Moscow 119017</p></bio><email xlink:type="simple">info@esoil.ru</email><xref ref-type="aff" rid="aff-1"/></contrib><contrib contrib-type="author" corresp="yes"><name-alternatives><name name-style="eastern" xml:lang="ru"><surname>Яшин</surname><given-names>М. А.</given-names></name><name name-style="western" xml:lang="en"><surname>Yashin</surname><given-names>M. A.</given-names></name></name-alternatives><bio xml:lang="ru"><p>119017, Москва, Пыжевский пер, 7, стр. 2</p></bio><bio xml:lang="en"><p>7 Bld. 2 Pyzhevskiy per., Moscow 119017</p></bio><email xlink:type="simple">info@esoil.ru</email><xref ref-type="aff" rid="aff-1"/></contrib><contrib contrib-type="author" corresp="yes"><name-alternatives><name name-style="eastern" xml:lang="ru"><surname>Хохлов</surname><given-names>С. Ф.</given-names></name><name name-style="western" xml:lang="en"><surname>Khokhlov</surname><given-names>S. F.</given-names></name></name-alternatives><bio xml:lang="ru"><p>119017, Москва, Пыжевский пер, 7, стр. 2</p></bio><bio xml:lang="en"><p>7 Bld. 2 Pyzhevskiy per., Moscow 119017</p></bio><email xlink:type="simple">info@esoil.ru</email><xref ref-type="aff" rid="aff-1"/></contrib><contrib contrib-type="author" corresp="yes"><name-alternatives><name name-style="eastern" xml:lang="ru"><surname>Ильин</surname><given-names>Б. С.</given-names></name><name name-style="western" xml:lang="en"><surname>Iliyn</surname><given-names>B. S.</given-names></name></name-alternatives><bio xml:lang="ru"><p>305526, Курская область, Курский район, п. Черeмушки, 10.</p></bio><bio xml:lang="en"><p>10 p. Cheremushki, Kursk region 305526</p></bio><email xlink:type="simple">kurskfarc@mail.ru</email><xref ref-type="aff" rid="aff-2"/></contrib><contrib contrib-type="author" corresp="yes"><name-alternatives><name name-style="eastern" xml:lang="ru"><surname>Лазарев</surname><given-names>В. И.</given-names></name><name name-style="western" xml:lang="en"><surname>Lazarev</surname><given-names>V. I.</given-names></name></name-alternatives><bio xml:lang="ru"><p>305526, Курская область, Курский район, п. Черeмушки, 10.</p></bio><bio xml:lang="en"><p>10 p. Cheremushki, Kursk region 305526</p></bio><email xlink:type="simple">kurskfarc@mail.ru</email><xref ref-type="aff" rid="aff-2"/></contrib><contrib contrib-type="author" corresp="yes"><name-alternatives><name name-style="eastern" xml:lang="ru"><surname>Холодов</surname><given-names>В. А.</given-names></name><name name-style="western" xml:lang="en"><surname>Kholodov</surname><given-names>V. A.</given-names></name></name-alternatives><bio xml:lang="ru"><p>119017, Москва, Пыжевский пер, 7, стр. 2</p></bio><bio xml:lang="en"><p>7 Bld. 2 Pyzhevskiy per., Moscow 119017</p></bio><email xlink:type="simple">info@esoil.ru</email><xref ref-type="aff" rid="aff-1"/></contrib></contrib-group><aff-alternatives id="aff-1"><aff xml:lang="ru">ФИЦ "Почвенный институт имени В.В. Докучаева"<country>Россия</country></aff><aff xml:lang="en">Federal Research Centre “V.V. Dokuchaev Soil Science Institute”<country>Russian Federation</country></aff></aff-alternatives><aff-alternatives id="aff-2"><aff xml:lang="ru">Курский федеральный научный центр<country>Россия</country></aff><aff xml:lang="en">Kursk Federal Agrarian Science Centre<country>Russian Federation</country></aff></aff-alternatives><pub-date pub-type="collection"><year>2020</year></pub-date><pub-date pub-type="epub"><day>28</day><month>09</month><year>2020</year></pub-date><volume>0</volume><issue>103</issue><fpage>85</fpage><lpage>107</lpage><permissions><copyright-statement>Copyright &amp;#x00A9; Фарходов Ю.Р., Ярославцева Н.В., Яшин М.А., Хохлов С.Ф., Ильин Б.С., Лазарев В.И., Холодов В.А., 2020</copyright-statement><copyright-year>2020</copyright-year><copyright-holder xml:lang="ru">Фарходов Ю.Р., Ярославцева Н.В., Яшин М.А., Хохлов С.Ф., Ильин Б.С., Лазарев В.И., Холодов В.А.</copyright-holder><copyright-holder xml:lang="en">Farkhodov Y.R., Yaroslavtseva N.V., Yashin M.A., Khokhlov S.F., Iliyn B.S., Lazarev V.I., Kholodov V.A.</copyright-holder><license license-type="creative-commons-attribution" xlink:href="https://creativecommons.org/licenses/by/4.0/" xlink:type="simple"><license-p>This work is licensed under a Creative Commons Attribution 4.0 License.</license-p></license></permissions><self-uri xlink:href="https://bulletin.esoil.ru/jour/article/view/577">https://bulletin.esoil.ru/jour/article/view/577</self-uri><abstract><p>Одним из наиболее обоснованных и востребованных подходов к выделению пулов органического вещества (ОВ) почвы является фракционирование с использованием тяжелых жидкостей. Проблема такого подхода состоит в достаточно больших потерях на стадии отмывки минеральной фракций от тяжелой жидкости, для преодоления этого затруднения предложено использовать вместо дистиллированной воды слабый раствор соляной кислоты. Выявлено, что такой подход позволит снизить потери почвенных частиц с 15 до 5% и потери углерода с 7.5 до 2.5%. В работе приводится подробный протокол денсиметрического фракционирования с помощью растворов поливольфрамата натрия, адаптированный для выделения из типичных черноземов четырех денсиметрических фракций: свободного и окклюдированного ОВ с плотностью &lt;1.6 г/см3, окклюдированного ОВ – 1.6–2.0 г/см3 и минеральной фракции – &gt;2.0 г/см3. Для проведения денсиметрического фракционирования использовали образцы типичных черноземов различного землепользования. Было показано, что процессы восстановления и деградации почв существенно сказываются на содержании легкого окклюдированного почвенного ОВ.</p></abstract><trans-abstract xml:lang="en"><p>One of the most justified and applied approaches to isolating pools of soil organic matter is fractionation in heavy liquids. The main problem with this approach is rather large losses in the separation of fractions at the stage of washing fractions from heavy liquids. The paper presents a densimetric fractionation protocol that can significantly reduce these losses. It is suggested to use 0.001 M HCl for washing. This approach, in comparison with distilled water, allows reducing losses of weight from 15 to 5% and of carbon from 7.5 to 2.5%. The paper provides a detailed protocol, used by the Laboratory of Soil Biochemistry of V.V. Dokuchaev Soil Science Institute, to isolate four densimetric fractions using sodium polytungstate solutions: free and occluded SOM with a density of &lt;1.6 g/cm3, occluded SOM – of 1.6–2.0 g/cm3, and a mineral residue with a density &gt;2.0 g/cm3. In the work we used samples of typical chernozems of different land use types. It was shown that the processes of soil restoration and degradation significantly affect the content of light occluded soil organic matter.</p></trans-abstract><kwd-group xml:lang="ru"><kwd>протокол выделения</kwd><kwd>многолетние опыты</kwd><kwd>почвенное органическое вещество</kwd><kwd>Haplic Chernozem</kwd><kwd>денсиметрическое фракционирование</kwd><kwd>поливольфрамат натрия</kwd></kwd-group><kwd-group xml:lang="en"><kwd>densimetric fractionation protocol</kwd><kwd>long-term experiments</kwd><kwd>soil organic matter</kwd><kwd>Haplic Chernozem</kwd><kwd>density separation</kwd><kwd>SPT</kwd><kwd>sodium polytungstate</kwd></kwd-group><funding-group xml:lang="ru"><funding-statement>РФФИ проект № 18-316-00101 мол_а , проект № 19-016-00078; Центр коллективного пользования научным оборудованием “Функции и свойства почв и почвенного покрова” Почвенного института им. В. В. Докучаева.</funding-statement></funding-group><funding-group xml:lang="en"><funding-statement>RFBR, project No. 18-316-00101 mol_a, project No. 19-016-00078; Centre for Collective Use of Scientific Equipment “Functions and Properties of Soils and Soil Cover” (V.V. Dokuchaev Soil Science Institute)</funding-statement></funding-group></article-meta></front><back><ref-list><title>References</title><ref id="cit1"><label>1</label><citation-alternatives><mixed-citation xml:lang="ru">Айдинян Р.Х. Краткая инструкция. Извлечение ила из почвы. М.: Гипроводхоз, 1960. 10 с.</mixed-citation><mixed-citation xml:lang="en">Aydinyan R.Kh., Kratkaya instruktsiya. Izvlecheniye ila iz pochvy (Brief instructions. Removing silt from the soil), Moscow: Giprovodkhoz, 1960, 10 p.</mixed-citation></citation-alternatives></ref><ref id="cit2"><label>2</label><citation-alternatives><mixed-citation xml:lang="ru">Когут Б.М., Сысуев С.А., Холодов В.А. 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