esoilБюллетень Почвенного института имени В.В. ДокучаеваDokuchaev Soil Bulletin0136-16942312-4202V.V. Dokuchaev Soil Science Institute10.19047/0136-1694-2024-SPYC-126-163esoil-844Research ArticleСпецвыпуск по результатам молодежной конференцииSpecial Issue "Selected papers from Young Scientists Conference 2022"Трансформация биохимических процессов в почве при загрязнении лекарственными препаратамиTransformation of biochemical processes in soil due to contamination by pharmaceuticalsТрегубоваП. Н.TregubovaP. N.

Полина Николавена Трегубова

121205, Москва, Большой бульвар, д.30, стр.1

1 bld. 30 Bolshoi Boulevard, Moscow 121205

p.tregubova@skoltech.ruАброськинД. П.AbroskinD. P.

Дмитрий Павлович Аброськин

121205, Москва, Большой бульвар, д.30, стр.1

1 bld. 30 Bolshoi Boulevard, Moscow 121205

dmitry.abroskin@skoltech.ruВолковаЕ. А.VolkovaE. A.

Елизавета Александровна Волкова

119991, Москва, Ленинские горы, 1

1 Leninskie Gori, Moscow 119234

lizvolk@inbox.ruЖеребкерА. Я.ZherebkerA. Y.

Александр Яковлевич Жеребкер

121205, Москва, Большой бульвар, д.30, стр.1

1 bld. 30 Bolshoi Boulevard, Moscow 121205

zherebker@gmail.comНиколаевЕ. Н.NikolaevE. N.

121205, Москва, Большой бульвар, д.30, стр.1

1 bld. 30 Bolshoi Boulevard, Moscow 121205

Сколковский институт науки и технологийРоссияSkolkovo Institute of Science and TechnologyRussian FederationМГУ им. М.В. ЛомоносоваРоссияLomonosov Moscow State UniversityRussian Federation20243011202400Специальный выпуск по материалам VII конференции молодых ученых “Почвоведение: Горизонты будущего. 2023"126163Copyright © Трегубова П.Н., Аброськин Д.П., Волкова Е.А., Жеребкер А.Я., Николаев Е.Н., 20242024Трегубова П.Н., Аброськин Д.П., Волкова Е.А., Жеребкер А.Я., Николаев Е.Н.Tregubova P.N., Abroskin D.P., Volkova E.A., Zherebker A.Y., Nikolaev E.N.Данная работа распространяется под лицензией Creative Commons Attribution 4.0.This work is licensed under a Creative Commons Attribution 4.0 License.https://bulletin.esoil.ru/jour/article/view/844

Лекарственные препараты и средства для личной гигиены (ЛППГ) являются новым типом загрязняющих веществ, регулярно детектируемых в природных средах, в первую очередь, в пресноводных источниках и сточных водах. В настоящее время собрана обширная база случаев обнаружения ЛППГ и в почвах, и проблема загрязнения данными эмерджентными веществами особенно актуальна для агроландшафтов. Целью исследования была оценка влияния ряда ЛППГ на процессы, протекающие в ризосфере на примере тестового растения. При проведении исследования предполагали, что попадание выбранных ЛППГ в почву может изменять биохимические процессы в ризосфере, влияя на активность микробного сообщества, в связи с чем могут изменяться характеристики лабильного пула органического вещества. В краткосрочном инкубационном эксперименте на гумусовом горизонте чернозема миграционно-мицелярного, исследовали влияние от ЛППГ, относящихся к наиболее актуальным терапевтическим группам и обнаруженных в природных средах: ципрофлоксацин (антибиотик), кетопрофен (анальгетик),  атенолол (бета-блокатор), клотримазол (антигрибковое средство). Препараты вносили в почву в градиенте концентраций; в качестве модельного растения использовали салат латук (Lactúca satíva). По окончании эксперимента значимым образом отличались от контроля биомасса побегов и корней, показатели ферментативной активности. Преимущественно отличия от контрольных образцов выражались в ингибирующем эффекте, в то же время в ряде случаев наблюдалось повышение показателей. Отмечено изменение состава растворенного органического вещества почвы ризосферы: по общему набору показателей от контрольного образца отличались варианты со вносом препаратов даже в низких концентрациях для ципрофлоксацина, кетопрофена и атенолола. Наблюдаемый эффект от внесения лекарственных препаратов можно объяснить как буферной емкостью исследованной почвы, так и биологической активностью почв, в частности, ризосферного сообщества, в то время как изменения носили нерегулярный характер.

Pharmaceuticals and personal care products (PPCPs) are emerging contaminants widely detected in natural environments, primarily in freshwater sources and wastewater. Currently, an extensive database of cases of detection of PPCPs in soils has been collected, and the problem of pollution with these emerging substances is especially relevant for agricultural landscapes. The study aimed to assess the influence of selected PPCPs on the processes occurring in the soils, especially in the rhizosphere, on the example of a test plant. As a working hypothesis, it was suggested that the introduction of selected PPCPs could change the biochemical processes in the rhizosphere, affecting the activity of the microbial community and, therefore, the characteristics of the labile pool of soil organic matter represented by watersoluble forms could change. In a short-term incubation experiment on the humus horizon of chernozem soil, we studied the effect of PPCPs represented by the most frequently prescribed therapeutic groups and often found in natural environments: ciprofloxacin (antibiotic), ketoprofen (analgesic), atenolol (beta-blocker), clotrimazole (antifungal agent). Selected substances were applied to the soil in concentration gradients. Lettuce (Lactúca sativa L.) was used as a test plant. At the end of the experiment, the biomass of shoots and roots and urease enzymatic activity in variants treated by PPCPs differed significantly from the control. Mainly, differences from control samples were expressed in the inhibitory effect; at the same time, an increase in indicators values was observed in several cases. A change in the composition of dissolved organic matter in the rhizosphere soil was noted: in general, even in low concentrations, samples treated with ciprofloxacin, ketoprofen, and atenolol differed from the control variant. While the changes were irregular in terms of concentrations of PPCPs, the observed effects caused by the introduction of selected substances can be explained by both the studied soil’s buffer capacity and the soil’s biological activity, particularly the rhizosphere community.

загрязнение почвэмерджентные загрязняющие веществарастворенное органическое веществоферментативная активностьмасс-спектрометрияsoil pollutionemerging pollutantsdissolved organic matterenzymatic activitymass spectrometryИсследование выполнено за счет гранта Российского научного фонда № 22-24-01103, https://rscf.ru/project/22-24-01103/The research was supported by the Russian Science Foundation grant No. 22-24-01103, https://rscf.ru/project/22-24-01103/ReferencesВодяницкий Ю.Н., Кириллова Н.П. Загрязнение почв и растений эмерджентными микрополлютантами // Агрохимия. 2016. № 5. С. 86–94.Vodyanitskii Yu.N., Kirillova, N.P., Zagryaznenie pochv i rastenii emerdzhentnymi mikropollyutantami (Contamination of soils and plants by emerging micropollutants), Agrokhimiya, 2016, No. 5, pp. 86–94.Жеребкер А.Я., Перминова И.В., Константинов А.И., Воликов А.Б., Костюкевич Ю.И., Кононихин А.С., Николаев Е.Н. Выделение гуминовых веществ из пресных вод на твердофазных картриджах и их исследование методом масс-спектрометрии ионно-циклотронного резонанса с преобразованием Фурье // Журнал аналитической химии. 2016. Т. 71. № 4(71). С. 390–396.Zherebker A.Ya., Perminova I.V., Konstantinov A.I., Volikov A.B., Kostyukevich Yu.I., Kononikhin A.S., Nikolaev E.N., Vydelenie guminovykh veshchestv iz presnykh vod na tverdofaznykh kartridzhakh i ikh issledovanie metodom mass-spektrometrii ionno-tsiklotronnogo rezonansa s preobrazovaniem Fur'e (Isolation of humic substances from fresh waters on solid-phase cartridges and their study by Fourier transform ion cyclotron resonance mass spectrometry), Zhurnal analiticheskoi khimii, 2016, Vol. 71, No. 4(71), pp. 390–396.Макаров М.И., Малышева Т.И., Маслов М.Н., Кузнецова Е.Ю., Меняйло О.В. Углерод и азот микробной биомассы в почвах южной тайги при определении разными методами // Почвоведение. 2016. № 6. С. 733– 744.Makarov M.I., Malysheva T.I., Maslov M.N., Kuznetsova E.Yu., Menyailo O.V., Uglerod i azot mikrobnoi biomassy v pochvakh yuzhnoi taigi pri opredelenii raznymi metodami (Carbon and nitrogen of microbial biomass in southern taiga soils when determined by different methods), Pochvovedenie, 2016, No. 6, pp. 733–744.Макаров М.И., Шулева М.С., Малышева Т.И., Меняйло О.В. Растворимость лабильных форм углерода и азота почв в K2SO4 разной концентрации // Почвоведение. 2013. № 4. С. 408–413.Makarov M.I., Shuleva M.S., Malysheva T.I., Menyailo O.V., Rastvorimost' labil'nykh form ugleroda i azota pochv v K2SO4 raznoi kontsentratsii (Solubility of labile forms of soil carbon and nitrogen in K2SO4 of various concentrations), Pochvovedenie, 2013, No. 4, pp. 408–413.Семенов М.В., Манучарова Н.А., Степанов А.Л. Распределение метаболически активных представителей прокариот (архей и бактерий) по профилям чернозема и бурой полупустынной почвы // Почвоведение. 2016. № 2. С. 239–248.Semenov M.V., Manucharova N.A., Stepanov A.L., Raspredelenie metabolicheski aktivnykh predstavitelei prokariot (arkhei i bakterii) po profilyam chernozema i buroi polupustynnoi pochvy (Distribution of metabolically active representatives of prokaryotes (archaea and bacteria) across the profiles of chernozem and brown semi-desert soil), Pochvovedenie, 2016, No. 2, pp. 239–248.Холодов В.А., Рогова О.Б., Лебедева М.П., Варламов Е.Б., Волков Д.С., Зиганшина А.Р., Ярославцева Н.В. Органическое вещество и минеральная матрица почв: современные подходы, определения терминов и методы изучения (обзор) // Бюллетень Почвенного института им. В.В. Докучаева. 2023. Вып. 117. С. 52–100. DOI: 10.19047/0136-1694-2023-117-52-100.Kholodov V.A., Rogova O.B., Lebedeva M.P., Varlamov E.B., Volkov D.S., Ziganshina A.R., Yaroslavtseva N.V., Organic matter and mineral matrix of soils: modern approaches, definitions of terms and methods of study (review), Dokuchaev Soil Bulletin, 2023, Vol. 117, pp. 52–100, DOI: 10.19047/0136-1694-2023-117-52-100.Arun S., Kumar R.M., Ruppa J., Mukhopadhyay M., Ilango K., Chakraborty P. Occurrence, sources and risk assessment of fluoroquinolones in dumpsite soil and sewage sludge from Chennai, India // Environmental Toxicology and Pharmacology. 2020. Vol. 79. DOI: 10.1016/j.etap.2020.103410.Arun S., Kumar R.M., Ruppa J., Mukhopadhyay M., Ilango K., Chakraborty P., Occurrence, sources and risk assessment of fluoroquinolones in dumpsite soil and sewage sludge from Chennai, India, Environmental Toxicology and Pharmacology, 2020, Vol. 79, DOI: 10.1016/j.etap.2020.103410.Bahureksa W., Tfaily M.M., Boiteau R.M., Young R.B., Logan M.N., McKenna A.M., Borch T. Soil Organic Matter Characterization by Fourier Transform Ion Cyclotron Resonance Mass Spectrometry (FTICR MS): A Critical Review of Sample Preparation, Analysis, and Data Interpretation // Environmental Science and Technology. 2021. Vol. 55(14). P. 9637–9656. DOI: 10.1021/acs.est.1c01135.Bahureksa W., Tfaily M.M., Boiteau R.M., Young R.B., Logan M.N., McKenna A.M., Borch T., Soil Organic Matter Characterization by Fourier Transform Ion Cyclotron Resonance Mass Spectrometry (FTICR MS): A Critical Review of Sample Preparation, Analysis, and Data Interpretation, Environmental Science and Technology, 2021, Vol. 55(14), pp. 9637–9656, DOI: 10.1021/acs.est.1c01135.Banerjee S., Mazumdar S. Electrospray ionization mass spectrometry: a technique to access the information beyond the molecular weight of the analyte // International Journal of Analytical Chemistry. 2012. Vol. 2012. DOI: 10.1155/2012/282574 .Banerjee S., Mazumdar S., Electrospray ionization mass spectrometry: a technique to access the information beyond the molecular weight of the analyte, International Journal of Analytical Chemistry, 2012, Vol. 2012, DOI: 10.1155/2012/282574.Blagodatskaya E., Kuzyakov Y. Active microorganisms in soil: critical review of estimation criteria and approaches // Soil Biology and Biochemistry. 2013. Vol. 67. P. 192–211. DOI: 10.1016/j.soilbio.2013.08.024.Blagodatskaya E., Kuzyakov Y., Active microorganisms in soil: critical review of estimation criteria and approaches, Soil Biology and Biochemistry, 2013, Vol. 67, pp. 192–211, DOI: 10.1016/j.soilbio.2013.08.024.Bolan N.S. Adriano D.C., Kunhikrishnan A., James T., Mcdowell R., Senesi N. Dissolved organic matter: biogeochemistry, dynamics, and environmental significance in soils // Advances in Agronomy. 2011. Vol. 110. P. 1–75. DOI: 10.1016/B978-0-12-385531-2.00001-3.Bolan N.S., Adriano D.C., Kunhikrishnan A., James T., Mcdowell R., Senesi N., Dissolved organic matter: biogeochemistry, dynamics, and environmental significance in soils, Advances in Agronomy, 2011, Vol. 110, pp. 1–75, DOI: 10.1016/B978-0-12-385531-2.00001-3.Chen Z., Zhang Y., Gao Y., Boyd S.A., Zhu D., Li H. Influence of Dissolved Organic Matter on Tetracycline Bioavailability to an Antibiotic-Resistant Bacterium // Environmental Science and Technology. 2015. Vol. 49(18). P. 10903–10910. DOI: 10.1021/acs.est.5b02158.Chen Z., Zhang Y, Gao Y, Boyd S.A., Zhu D., Li H., Influence of Dissolved Organic Matter on Tetracycline Bioavailability to an AntibioticResistant Bacterium, Environmental Science and Technology, 2015, Vol. 49(18), pp. 10903–10910, DOI: 10.1021/acs.est.5b02158.Chinemerem Nwobodo D., Ugwu M.C., Oliseloke Anie C., Al-Ouqaili M.T., Chinedu Ikem J., Victor Chigozie U., Saki M. Antibiotic resistance: The challenges and some emerging strategies for tackling a global menace // Journal of clinical laboratory analysis. 2022. Vol. 36(9). DOI: 10.1002/jcla.24655.Chinemerem Nwobodo, D., Ugwu M.C., Oliseloke A.C., Al-Ouqaili M.T., Chinedu Ikem J., Victor Chigozie U., Saki M., Antibiotic resistance: The challenges and some emerging strategies for tackling a global menace, Journal of clinical laboratory analysis, 2022, Vol. 36(9), DOI: 10.1002/jcla.24655.Daughton C.G., Ternes T.A. Pharmaceuticals and personal care products in the environment: agents of subtle change? // Environmental health perspectives. 1999. Vol. 107(6). P. 907–938. DOI: 10.1289/ehp.99107s6907.Daughton C.G., Ternes T.A. Pharmaceuticals and personal care products in the environment: agents of subtle change? Environmental health perspectives, 1999, Vol. 107(6), pp. 907–938, DOI: 10.1289/ehp.99107s6907.Drzymala J., Kalka J. Assessment of genotoxicity, mutagenicity, and cytotoxicity of diclofenac and sulfamethoxazole at environmental concentrations on Vicia faba // International Journal of Environmental Science and Technology. 2024. Vol. 21. P. 3633–3648. DOI: 10.1007/s13762-02305238-4.Drzymala J., Kalka, J., Assessment of genotoxicity, mutagenicity, and cytotoxicity of diclofenac and sulfamethoxazole at environmental concentrations on Vicia faba, International Journal of Environmental Science and Technology, 2024, Vol. 21, pp. 3633–3648, DOI: 10.1007/s13762-023-05238-4.Ebele A.J., Abdallah M.A.-E., Harrad S. Pharmaceuticals and personal care products (PPCPs) in the freshwater aquatic environment // Emerging Contaminants. 2017. Vol. 3(1). P. 1–16. DOI: 10.1016/j.emcon.2016.12.004.Ebele A.J., Abdallah M.A.-E., Harrad S., Pharmaceuticals and personal care products (PPCPs) in the freshwater aquatic environment, Emerging Contaminants, 2017, Vol. 3(1), pp. 1–16, DOI: 10.1016/j.emcon.2016.12.004.Frkova Z., Vystavna Y., Koubová A., Kotas P., Grabicová K., Grabic R., Kodešová R., Chroňáková A. Microbial responses to selected pharmaceuticals in agricultural soils: Microcosm study on the roles of soil, treatment and time // Soil Biology and Biochemistry. 2020. Vol. 149. DOI: 10.1016/j.soilbio.2020.107924.Frkova Z., Vystavna Y., Koubová A., Kotas P., Grabicová K., Grabic R., Kodešová R., Chroňáková A., Microbial responses to selected pharmaceuticals in agricultural soils: Microcosm study on the roles of soil, treatment and time, Soil Biology and Biochemistry, 2020, Vol. 149, DOI: 10.1016/j.soilbio.2020.107924.Gallego S., Brienza M., Béguet J., Chiron S., Martin-Laurent F. Impact of repeated irrigation of lettuce cultures with municipal wastewater on soil bacterial community diversity and composition // Environmental Science and Pollution Research. 2022. Vol. 29(20). P. 29236–29243. DOI: 10.1007/s11356-021-14734-4.Gallego S., Brienza M., Béguet J., Chiron S., Martin-Laurent F., Impact of repeated irrigation of lettuce cultures with municipal wastewater on soil bacterial community diversity and composition, Environmental Science and Pollution Research, 2022, Vol. 29(20), pp. 29236–29243, DOI: 10.1007/s11356-021-14734-4.Gallego S., Montemurro N., Béguet J., Rouard N., Philippot L., Pérez S., Martin-Laurent F. Ecotoxicological risk assessment of wastewater irrigation on soil microorganisms: Fate and impact of wastewater-borne micropollutants in lettuce-soil system // Ecotoxicology and Environmental Safety. 2021. Vol. 223. DOI: 10.1016/j.ecoenv.2021.112595.Gallego S., Montemurro N., Béguet J., Rouard N., Philippot L., Pérez S., Martin-Laurent F., Ecotoxicological risk assessment of wastewater irrigation on soil microorganisms: Fate and impact of wastewater-borne micropollutants in lettuce-soil system, Ecotoxicology and Environmental Safety, 2021, Vol. 223, DOI:10.1016/j.ecoenv.2021.112595.González Peña O.I., López Zavala M.A., Cabral Ruelas H. Pharmaceuticals Market, Consumption Trends and Disease Incidence Are Not Driving the Pharmaceutical Research on Water and Wastewater // International Journal of Environmental Research and Public Health. 2021. Vol. 18(5). DOI: 10.3390/ijerph18052532.Gonzalez Pena O.I., Lopez Zavala M.A., Cabral Ruelas H., Pharmaceuticals market, consumption trends and disease incidence are not driving the pharmaceutical research on water and wastewater, International Journal of Environmental Research and Public Health, 2021, Vol. 18(5), DOI: 10.3390/ijerph18052532.Graumnitz S., Jungmann D. The database “pharmaceuticals in the environment” – update and new analysis. GWT-TUD GmbH, Dresden, 2021.Graumnitz S., Jungmann D., The database “Pharmaceuticals in the environment”-update and new analysis, GWT-TUD GmbH, Dresden, 2021.Grossberger A., Hadar Y., Borch T., Chefetz B. Biodegradability of pharmaceutical compounds in agricultural soils irrigated with treated wastewater // Environmental pollution. 2014. Vol. 185. P. 168–177. DOI: 10.1016/j.envpol.2013.10.038.Grossberger A., Hadar Y., Borch T., Chefetz B., Biodegradability of pharmaceutical compounds in agricultural soils irrigated with treated wastewater, Environmental pollution, 2014, Vol. 185, pp. 168–177, DOI: 10.1016/j.envpol.2013.10.038.Hossain A., Krupnik T.J., Timsina J., Mahboob M.G., Chaki A.K., Farooq M., Hasanuzzaman M. Chapter – 2 Agricultural Land Degradation: Processes and Problems Undermining Future Food Security, In: Environment, Climate, Plant and Vegetation Growth (First Edition). Springer, 2020. P. 17– 61.Hossain A., Krupnik T.J., Timsina J., Mahboob M.G., Chaki A.K., Farooq M., Hasanuzzaman M., Chapter – 2 Agricultural Land Degradation: Processes and Problems Undermining Future Food Security, In: Environment, Climate, Plant and Vegetation Growth (First Edition), Springer, 2020, pp. 17– 61.Hou J., Wan W., Mao D., Wang C., Mu Q., Qin S., Luo Y. Occurrence and distribution of sulfonamides, tetracyclines, quinolones, macrolides, and nitrofurans in livestock manure and amended soils of Northern China // Environmental Science and Pollution Research. 2015. Vol. 22(6). P. 4545– 4554. DOI: 10.1007/s11356-014-3632-y.Hou J., Wan W., Mao D., Wang C., Mu Q., Qin S., Luo Y., Occurrence and distribution of sulfonamides, tetracyclines, quinolones, macrolides, and nitrofurans in livestock manure and amended soils of Northern China, Environmental Science and Pollution Research, 2015, Vol. 22(6), pp. 4545– 4554, DOI: 10.1007/s11356-014-3632-y.Kuzyakov Y., Blagodatskaya E. Microbial hotspots and hot moments in soil: concept & review // Soil Biology and Biochemistry. 2015. Vol. 83. P. 184–199. DOI: 10.1016/j.soilbio.2015.01.025.Kuzyakov Y., Blagodatskaya E., Microbial hotspots and hot moments in soil: concept & review, Soil Biology and Biochemistry, 2015, Vol. 83, pp. 184–199, DOI: 10.1016/j.soilbio.2015.01.025.Lagos S., Tsetsekos G., Mastrogianopoulos S., Tyligada M., Diamanti L., Vasileiadis S., Sotiraki S., Karpouzas D.G. Interactions of anthelmintic veterinary drugs with the soil microbiota: Toxicity or enhanced biodegradation? // Environmental Pollution. 2023. Vol. 334. DOI: 10.1016/j.envpol.2023.122135.Lagos S., Tsetsekos G., Mastrogianopoulos S., Tyligada M., Diamanti L., Vasileiadis S., Sotiraki S., Karpouzas D.G., Interactions of anthelmintic veterinary drugs with the soil microbiota: Toxicity or enhanced biodegradation? Environmental Pollution, 2023, Vol. 334, DOI: 10.1016/j.envpol.2023.122135.Leal R.M.P., Figueira R.F., Tornisielo V.L., Regitano J.B. Occurrence and sorption of fluoroquinolones in poultry litters and soils from São Paulo State, Brazil // The Science of The Total Environment. 2012. Vol. 432. P. 344–349. DOI: 10.1016/j.scitotenv.2012.06.002.Leal R.M.P., Figueira R.F., Tornisielo V.L., Regitano J.B., Occurrence and sorption of fluoroquinolones in poultry litters and soils from São Paulo State, Brazil, The Science of The Total Environment, 2012, Vol. 432, pp. 3445–349, DOI: 10.1016/j.scitotenv.2012.06.002.Lehmann, J., Bossio, D.A., Kögel-Knabner, I., Rillig, M.C. The concept and future prospects of soil health // Nature Reviews. Earth & Environment. 2020. Vol. 1(10). P. 544–553. DOI: 10.1038/s43017-020-0080-8.Lehmann J., Bossio D.A., Kögel-Knabner I., Rillig M.C., The concept and future prospects of soil health, Nature Reviews. Earth & Environment, 2020, Vol. 1(10), pp. 544–553, DOI: 10.1038/s43017-020-0080-8.Li, X., He, F., Wang, Z., Xing, B. Roadmap of environmental health research on emerging contaminants: Inspiration from the studies on engineered nanomaterials // Eco-Environment & Health. 2022. Vol. 1(3). P. 181-197. DOI: 10.1016/j.eehl.2022.10.001.Li X., He F., Wang Z., Xing B., Roadmap of environmental health research on emerging contaminants: Inspiration from the studies on engineered nanomaterials, Eco-Environment & Health, 2022, Vol. 1(3), pp. 1815–197, DOI: 10.1016/j.eehl.2022.10.001.Li, J., Wang, C., Liang, W., Liu, S. Rhizosphere Microbiome: The Emerging Barrier in Plant-Pathogen Interactions // Frontiers in Microbiology. 2021. Vol. 12. DOI: 10.3389/fmicb.2021.772420.Li J., Wang C., Liang W., Liu S., Rhizosphere Microbiome: The Emerging Barrier in Plant-Pathogen Interactions, Frontiers in Microbiology, 2021, Vol. 12, DOI: 10.3389/fmicb.2021.772420.Ma, T., Pan, X., Liu, W., Christie, P., Luo, Y., Wu, L., et al. Effects of different concentrations and application frequencies of oxytetracycline on soil enzyme activities and microbial community diversity // European Journal of Soil Biology. 2016. Vol. 76. P. 53–60. DOI: 10.1016/j.ejsobi.2016.07.004.Ma T., Pan X., Liu W., Christie P., Luo Y., Wu L., et al., Effects of different concentrations and application frequencies of oxytetracycline on soil enzyme activities and microbial community diversity, European Journal of Soil Biology, 2016, Vol. 76, pp. 53–60, DOI: 10.1016/j.ejsobi.2016.07.004.McKinsey & Company. The future of wellness: Connected and customized, 2021.McKinsey & Company, The future of wellness: Connected and customized, 2021.Meffe, R., de Santiago-Martín, A., Teijon, G., Hernandez, V.M., Lopez-Heras, I., Nozal, L., de Bustamante, I. Pharmaceutical and transformation products during unplanned water reuse: Insights into natural attenuation, plant uptake and human health impact under field conditions // Environment International. 2021. Vol. 157. DOI: 10.1016/j.envint.2021.106835.Meffe R., de SantiagoMartín A., Teijon G., Hernandez V.M., LopezHeras I., Nozal L., de Bustamante I., Pharmaceutical and transformation products during unplanned water reuse: Insights into natural attenuation, plant uptake and human health impact under field conditions, Environment International, 2021, Vol. 157, DOI: 10.1016/j.envint.2021.106835.Mendes, L.W., Raaijmakers, J.M., de Hollander, M., Mendes, R., Tsai, S.M. Influence of resistance breeding in common bean on rhizosphere microbiome composition and function // The ISME journal. 2018. Vol. 12(1). P. 212–224. DOI: 10.1038/ismej.2017.158.Mendes L.W., Raaijmakers J.M., de Hollander M., Mendes R., Tsai S.M., Influence of resistance breeding in common bean on rhizosphere microbiome composition and function, The ISME journal, 2018, Vol. 12(1), pp. 212–224, DOI: 10.1038/ismej.2017.158.Navrátilová M., Raisova Stuchlíková, L., Moťková, K., Szotáková, B., Skálová, L., Langhansová, L., Podlipná, R. The uptake of ivermectin and its effects in roots, leaves and seeds of soybean (glycine max) // Molecules. 2020. Vol. 25(16). DOI: 10.3390/molecules25163655.Navrátilová M., Raisova Stuchlíková L., Moťková K., Szotáková B., Skálová L., Langhansová L., Podlipná R., The uptake of ivermectin and its effects in roots, leaves and seeds of soybean (glycine max), Molecules, 2020, Vol. 25(16), DOI: 10.3390/molecules25163655.Parham J. A., Deng S. P. Detection, quantification and characterization of β-glucosaminidase activity in soil // Soil biology and biochemistry. 2000. Vol. 32(8-9). P. 1183-1190. DOI: 10.1016/S0038-0717(00)00034-1.Parham J.A., Deng S.P., Detection, quantification and characterization of β-glucosaminidase activity in soil, Soil biology and biochemistry, 2000, Vol. 32(8–9), pp. 1183–1190, DOI: 10.1016/S0038-0717(00)00034-1.Rodríguez Eugenio, N., McLaughlin, M., Pennock, D. Soil pollution: a hidden reality. Rome: FAO, 2018. 142 p.Rodriguez E.N., McLaughlin M., Pennock D., Soil pollution: a hidden reality, Rome: FAO, 2018, 142 p.Saleem, M., Hu, J., Jousset, A. More Than the Sum of Its Parts: Microbiome Biodiversity as a Driver of Plant Growth and Soil Health // Annual Review of Ecology, Evolution, and Systematics. 2019. Vol. 50(1). P. 145–168. DOI: 10.1146/annurev-ecolsys-110617-062605.Saleem M., Hu J., Jousset A., More Than the Sum of Its Parts: Microbiome Biodiversity as a Driver of Plant Growth and Soil Health, Annual Review of Ecology, Evolution, and Systematics, 2019, Vol. 50(1), pp. 145–168, DOI: 10.1146/annurev-ecolsys-110617-062605.Santás-Miguel V., Díaz-Raviña M., Martín A. García-Campos E., Barreiro A., Núñez-Delgado A., Álvarez-Rodríguez E., Arias-Estévez M., Fernández-Calviño D. Soil Enzymatic Activities and Microbial Community Structure in Soils Polluted with Tetracycline Antibiotics // Agronomy. 2021. Vol. 11(5). DOI: 10.3390/agronomy11050906.Santás-Miguel V., Díaz-Raviña M., Martín A., García-Campos E., Barreiro A., Núñez-Delgado A., Álvarez-Rodríguez E., Arias-Estévez M., Fernández-Calviño D., Soil Enzymatic Activities and Microbial Community Structure in Soils Polluted with Tetracycline Antibiotics, Agronomy, 2021, Vol. 11(5), DOI: 10.3390/agronomy11050906.Schinner F., Öhlinger R., Kandeler E., Margesin R. Methods in Soil Biology. Springer Berlin, Heidelberg, 1996. 426 p.Schinner F., Ohlinger R., Kandeler E., Margesin R., Methods in Soil Biology, Springer Berlin, Heidelberg, 1996, 426 p.Sokol N.W., Slessarev E., Marschmann G.L., Nicolas A., Blazewicz S.J., Brodie E.L., Firestone M.K., Foley M.M., Hestrin R., Hungate B.A., Koch B.J., Stone B.W., Sullivan M.B., Zablocki O. LLNL Soil Microbiome Consortium, Pett-Ridge J. Life and death in the soil microbiome: How ecological processes influence biogeochemistry // Nature Reviews Microbiology. 2022. Vol. 20(7). P. 415–430. DOI: 10.1038/s41579-02200695-z.Sokol N.W., Slessarev E., Marschmann G.L., Nicolas A., Blazewicz S.J., Brodie E.L., Firestone M.K., Foley M.M., Hestrin R., Hungate B.A., Koch B.J., Stone B.W., Sullivan M.B., Zablocki O., LLNL Soil Microbiome Consortium, Pett-Ridge J., Life and death in the soil microbiome: How ecological processes influence biogeochemistry, Nature Reviews Microbiology, 2022, Vol. 20(7), pp. 415–430, DOI: 10.1038/s41579-02200695-z.Tfaily M.M., Chu R.K., Toyoda J., Tolić N., Robinson E.W., Paša-Tolić L., Hess N.J. Sequential extraction protocol for organic matter from soils and sediments using high resolution mass spectrometry // Analytica Chimica Acta. 2017. Vol. 972. P. 54–61. DOI: 10.1016/j.aca.2017.03.031.Tfaily M.M., Chu R.K., Toyoda J., Tolić N., Robinson E.W., PašaTolić L., Hess N.J., Sequential extraction protocol for organic matter from soils and sediments using high resolution mass spectrometry, Analytica Chimica Acta, 2017, Vol. 972, pp. 54–61, DOI:10.1016/j.aca.2017.03.031.Tremblay L.B., Dittmar T., Marshall A.G. Cooper W.J., Cooper W.T. Molecular characterization of dissolved organic matter in a North Brazilian mangrove porewater and mangrove-fringed estuaries by ultrahigh resolution Fourier Transform-Ion Cyclotron Resonance mass spectrometry and excitation/emission spectroscopy // Marine Chemistry. 2007. Vol. 105(1–2). P. 15–29. DOI: 10.1016/J.Marchem.2006.12.015.Tremblay L.B., Dittmar T., Marshall A.G., Cooper W.J., Cooper W.T., Molecular characterization of dissolved organic matter in a North Brazilian mangrove porewater and mangrove-fringed estuaries by ultrahigh resolution Fourier Transform-Ion Cyclotron Resonance mass spectrometry and excitation/emission spectroscopy, Marine Chemistry, 2007, Vol. 105(1–2), pp. 15–29, DOI:10.1016/J.Marchem.2006.12.015.Volikov A., Rukhovich G., Perminova I. NOMspectra: An Open-Source Python Package for Processing High Resolution Mass Spectrometry Data on Natural Organic Matter // Journal of the American Society for Mass Spectrometry. 2023. Vol. 34(7). DOI: 10.1021/jasms.3c00003.Volikov A., Rukhovich G., Perminova I., NOMspectra: An Open-Source Python Package for Processing High Resolution Mass Spectrometry Data on Natural Organic Matter, Journal of the American Society for Mass Spectrometry, 2023, Vol. 34(7), DOI: 10.1021/jasms.3c00003.Wiesmeier M., Urbanski L., Hobley E., Lang B., Lützow M., Marin-Spiotta E., Wesemael B., Rabot E., Ließ M., Garcia-Franco N., Wollschläger U., Vogel H., Kögel‐ Knabner I. Soil Organic Carbon Storage as a Key Function of Soils: A Review of Drivers and Indicators at Various Scales // Geoderma. 2019. Vol. 333. P. 149–162. DOI: 10.1016/j.geoderma.2018.07.026.Wiesmeier M., Urbanski L., Hobley E., Lang B., Lützow M., MarinSpiotta E., Wesemael B., Rabot E., Ließ M., Garcia-Franco N., Wollschläger U., Vogel H., Kögel‐ Knabner I., Soil Organic Carbon Storage as a Key Function of Soils: A Review of Drivers and Indicators at Various Scales, Geoderma, 2019, Vol. 333, pp. 149–162, DOI: 10.1016/j.geoderma.2018.07.026.World Health Organization. Antimicrobial resistance: global report on surveillance. World Health Organization, 2014. 256 p.World Health Organization. Antimicrobial resistance: global report on surveillance, World Health Organization, 2014, 256 p.Zheng J., Zhang J., Gao L., Kong F., Shen G., Wang R., Gao J., Zhang J. The Effects of Tetracycline Residues on the Microbial Community Structure of Tobacco Soil in Pot Experiment // Scientific Reports. 2020. Vol. 10(1). DOI: 10.1038/s41598-020-65203-w.Zheng J., Zhang J., Gao L., Kong F., Shen G., Wang R., Gao J., Zhang J., The Effects of Tetracycline Residues on the Microbial Community Structure of Tobacco Soil in Pot Experiment, Scientific Reports, 2020, Vol. 10(1), DOI: 10.1038/s41598-020-65203-w.

The authors declare that there are no conflicts of interest present.