esoilБюллетень Почвенного института имени В.В. ДокучаеваDokuchaev Soil Bulletin0136-16942312-4202V.V. Dokuchaev Soil Science Institute10.19047/0136-1694-2025-124-254-271esoil-898Research ArticleСпециальный номер "Органическое вещество почв"Special Issue "Soil Organic Matter"Молекулярный состав водоэкстрагируемого органического вещества черноземов при выращивании ячменя в условиях вегетационного опытаMolecular composition of water-extractable organic matter in chernozem soils during barley cultivation in a vegetation experimenthttps://orcid.org/0000-0002-0210-380XФарходовЮ. Р.FarkhodovYu. R.

Юлиан Робертович Фарходов

119017, Москва, Пыжевский пер, 7, стр. 2

7 Bld. 2 Pyzhevskiy per., Moscow 119017

yulian.farkhodov@yandex.ruДанилинИ. В.DanilinI. V.

119017, Москва, Пыжевский пер, 7, стр. 2

7 Bld. 2 Pyzhevskiy per., Moscow 119017

ЯрославцеваН. В.YaroslavtsevaN. V.

119017, Москва, Пыжевский пер, 7, стр. 2

7 Bld. 2 Pyzhevskiy per., Moscow 119017

ЗиганшинаА. Р.ZiganshinaA. R.

119017, Москва, Пыжевский пер, 7, стр. 2

7 Bld. 2 Pyzhevskiy per., Moscow 119017

МаксимовичС. В.MaksimovichS. V.

119017, Москва, Пыжевский пер, 7, стр. 2

7 Bld. 2 Pyzhevskiy per., Moscow 119017

ХолодовВ. А.KholodovV. A.

119017, Москва, Пыжевский пер, 7, стр. 2

7 Bld. 2 Pyzhevskiy per., Moscow 119017

ФИЦ “Почвенный институт им. В.В. Докучаева”РоссияFederal Research Centre “V.V. Dokuchaev Soil Science Institute”Russian Federation2025200920250124"Почвенное органическое вещество"254271Copyright © Фарходов Ю.Р., Данилин И.В., Ярославцева Н.В., Зиганшина А.Р., Максимович С.В., Холодов В.А., 20252025Фарходов Ю.Р., Данилин И.В., Ярославцева Н.В., Зиганшина А.Р., Максимович С.В., Холодов В.А.Farkhodov Y.R., Danilin I.V., Yaroslavtseva N.V., Ziganshina A.R., Maksimovich S.V., Kholodov V.A.Данная работа распространяется под лицензией Creative Commons Attribution 4.0.This work is licensed under a Creative Commons Attribution 4.0 License.https://bulletin.esoil.ru/jour/article/view/898

Целью работы была оценка влияния вегетации ячменя (Hordeum vulgare L.) на молекулярный состав водоэкстрагируемого органического вещества черноземов. В работе использовали метод вегетационного эксперимента в климатической камере с отбором проб почвы до посева и во время вегетации ячменя. Молекулярный состав водоэкстрагируемого органического вещества (ВЭОВ) изучали методом газовой хромато-масс-спектрометрии. На основе полученных данных рассчитывали индекс разнообразия Шеннона и оценивали вклад разных соединений в состав ВЭОВ. Показано, что вегетация ячменя увеличивает сложность состава ВЭОВ чернозема. Молекулярный состав ВЭОВ оказался различным для всех вариантов опыта. Доля липидов и азотсодержащих соединений ВЭОВ чернозема в условиях вегетации ячменя уменьшается по сравнению с его предпосевным состоянием, что может быть связано с их активной микробной деструкцией. При вегетации ячменя происходит значимое увеличение доли углеводов в составе ВЭОВ чернозема. Полученные данные свидетельствуют о высокой чувствительности молекулярного состава ВЭОВ черноземов к влиянию функционирования ячменя и ризосферных микроорганизмов.

The aim of this study was to assess the impact of barley (Hordeum vulgare L.) vegetation on the molecular composition of water-extractable organic matter (WEOM) in chernozem soils. The study employed a vegetation experiment method in a climate chamber, with soil samples taken before sowing and during barley vegetation. The molecular composition of WEOM was analyzed using gas chromatography-mass spectrometry (GC-MS). Based on the obtained data, the Shannon diversity index was calculated, and the contribution of different compounds to the composition of WEOM was evaluated. It was shown that barley vegetation increases the complexity of the WEOM composition in chernozem. The molecular composition of WEOM varied for all experimental conditions. The proportion of lipids and nitrogen-containing compounds in WEOM of chernozem decreased during barley vegetation compared to its pre-sowing state, which may be associated with their active microbial decomposition. There was a significant increase in the proportion of carbohydrates in the WEOM composition of chernozem during barley vegetation. The obtained data indicate the high sensitivity of the molecular composition of WEOM in chernozem soils to the influence of barley functioning and rhizospheric microorganisms.

ячменьризосфераГХ/МСлабильное ПОВbarleyrhizosphereGC/MSlabile SOMИсследование выполнено при поддержке Российского научного фонда, проект № 23-26-00107This research was funded by the Russian Science Foundation (project No. 24- 26-00293), https://www.rscf.ru/project/24-26-00293ReferencesКлассификация и диагностика почв СССР / Под ред. Егорова В.В. и др. М: Колос, 1977. 235 с.Egorov V.V. et. al (Eds.), Klassifikatsiya i diagnostika pochv SSSR (Classification and Diagnostics of USSR Soils), Moscow: Kolos, 1977. 235 p.Нельсон Д., Кокс М. Основы биохимии Ленинджера. Лаборатория знаний, 2022. 636 с.Nelson D., Cox M., Osnovy biokhimii Lenindzhera (Principles of Biochemistry by Lehninger), Knowledge Laboratory, 2022. 636 p.Соколова Т.А. Роль биоты в создании почвенного профиля и функционировании почвы: новые материалы и интерпретация известных фактов и существующих концепций // Бюллетень Почвенного института им. В.В. Докучаева. 2020. Вып. 105. C. 208‒225. DOI: https://doi.org/10.19047/0136-1694-2020-105-208-225.Sokolova T.A., The role of biota in soil profile formation and soil functioning: new materials and interpretation of well-known facts and existing concepts, Dokuchaev Soil Bulletin, 2020, Vol. 105, pp. 208‒225, DOI: https://doi.org/10.19047/0136-1694-2020-105-208-225.Aitchison J. The statistical analysis of compositional data // Journal of the Royal Statistical Society: Series B (Methodological). 1982. Vol. 4. No. 2. P. 139‒160.Aitchison J., The statistical analysis of compositional data, Journal of the Royal Statistical Society: Series B (Methodological), 1982, Vol. 4, No. 2, pp. 139‒160.Aksenov A.A., Laponogov I., Zhang Z., Doran S.L.F., Belluomo I., Veselkov D., Bittremieux W., Nothias L. F., Nothias-Esposito M. et al. Autodeconvolution and molecular networking of gas chromatography–mass spectrometry data // Nature Biotechnology. 2021. Vol. 39. No. 2. P. 169‒173.Aksenov A.A., Laponogov I., Zhang Z., Doran S.L.F., Belluomo I., Veselkov D., Bittremieux W., Nothias L. F., Nothias-Esposito M. et al., Autodeconvolution and molecular networking of gas chromatography–mass spectrometry data, Nature Biotechnology, 2021, Vol. 39, No. 2, pp. 169‒173.Bahadori M., Chen C., Lewis S., Boyd S., Rashti M.R., Esfandbod M., Garzon-Garcia A., Van Zwieten L., Kuzyakov Y. Soil organic matter formation is controlled by the chemistry and bioavailability of organic carbon inputs across different land uses // Science of The Total Environment. 2021. Vol. 770. P. 145307.Bahadori M., Chen C., Lewis S., Boyd S., Rashti M.R., Esfandbod M., Garzon-Garcia A., Van Zwieten L., Kuzyakov Y., Soil organic matter formation is controlled by the chemistry and bioavailability of organic carbon inputs across different land uses, Science of The Total Environment, 2021, Vol. 770, pp. 145307.Chai Y.N., Schachtman D. P. Root exudates impact plant performance under abiotic stress // Trends in Plant Science. 2022. Vol. 27. No. 1. P. 80‒91.Chai Y.N., Schachtman D.P., Root exudates impact plant performance under abiotic stress, Trends in Plant Science, 2022, Vol. 27, No. 1, pp. 80‒91.Chen Y., Yao Z., Sun Y., Wang E., Tian C., Sun Y., Liu J., Sun C., Tian L. Current Studies of the Effects of Drought Stress on Root Exudates and Rhizosphere Microbiomes of Crop Plant Species // International Journal of Molecular Sciences. 2022. Vol. 23. No. 4. P. 2374.Chen Y., Yao Z., Sun Y., Wang E., Tian C., Sun Y., Liu J., Sun C., Tian L., Current Studies of the Effects of Drought Stress on Root Exudates and Rhizosphere Microbiomes of Crop Plant Species, International Journal of Molecular Sciences, 2022, Vol. 23, No. 4, pp. 2374.Dakora F.D., Phillips D.A. Root exudates as mediators of mineral acquisition in low-nutrient environments // Food Security in Nutrient-Stressed Environments: Exploiting Plants’ Genetic Capabilities. Dordrecht: Springer Netherlands, 2002. P. 201‒213.Dakora F.D., Phillips D.A., Root exudates as mediators of mineral acquisition in low-nutrient environments, In: Food Security in NutrientStressed Environments: Exploiting Plants’ Genetic Capabilities, Dordrecht: Springer Netherlands, 2002, pp. 201‒213.Dhungana I., Kantar M.B., Nguyen N.H. Root exudate composition from different plant species influences the growth of rhizosphere bacteria // Rhizosphere. 2023. Vol. 25. P. 100645.Dhungana I., Kantar M.B., Nguyen N.H., Root exudate composition from different plant species influences the growth of rhizosphere bacteria, Rhizosphere, 2023, Vol. 25, pp. 100645.dos Santos Castro L., Pedersoli W.R., Antoniêto A.C.C., Steindorff A.S., Silva-Rocha R., Martinez-Rossi N.M., Rossi A., Brown N.A., Goldman G.H., Faça V.M., Persinoti G.F., Silva R.N. Comparative metabolism of cellulose, sophorose and glucose in Trichoderma reeseiusing high-throughput genomic and proteomic analyses // Biotechnology for Biofuels. 2014. Vol. 7. No. 1. P. 41.dos Santos Castro L., Pedersoli W.R., Antoniêto A.C.C., Steindorff A.S., Silva-Rocha R., Martinez-Rossi N.M., Rossi A., Brown N.A., Goldman G.H., Faça V.M., Persinoti G.F., Silva R.N., Comparative metabolism of cellulose, sophorose and glucose in Trichoderma reeseiusing high-throughput genomic and proteomic analyses, Biotechnology for Biofuels, 2014, Vol. 7, No. 1, pp. 41.El Moujahid L., Le Roux X., Michalet S., Bellvert F., Weigelt A., Poly F. Effect of plant diversity on the diversity of soil organic compounds // PLOS ONE. 2017. Vol. 12. No. 2. P. e0170494.El Moujahid L., Le Roux X., Michalet S., Bellvert F., Weigelt A., Poly F., Effect of plant diversity on the diversity of soil organic compounds, PLOS ONE, 2017, Vol. 12, No. 2, pp. e0170494.Etesami H. Potential advantage of rhizosheath microbiome, in contrast to rhizosphere microbiome, to improve drought tolerance in crops // Rhizosphere. 2021. Vol. 20. P. 100439.Etesami H., Potential advantage of rhizosheath microbiome, in contrast to rhizosphere microbiome, to improve drought tolerance in crops, Rhizosphere, 2021, Vol. 20, pp. 100439.Fadiji A.E., Yadav A.N., Santoyo G., Babalola O.O. Understanding the plant-microbe interactions in environments exposed to abiotic stresses: An overview // Microbiological Research. 2023. Vol. 271. P. 127368.Fadiji A.E., Yadav A.N., Santoyo G., Babalola O.O., Understanding the plant-microbe interactions in environments exposed to abiotic stresses: An overview, Microbiological Research, 2023, Vol. 271, pp. 127368.Feng H., Fu R., Hou X., Lv Y., Zhang N., Liu Y., Xu Z., Miao Y., Krell T., Shen Q., Zhang R. Chemotaxis of Beneficial Rhizobacteria to Root Exudates: The First Step towards Root–Microbe Rhizosphere Interactions // International Journal of Molecular Sciences. 2021. Vol. 22. No. 13. P. 6655.Feng H., Fu R., Hou X., Lv Y., Zhang N., Liu Y., Xu Z., Miao Y., Krell T., Shen Q., Zhang R., Chemotaxis of Beneficial Rhizobacteria to Root Exudates: The First Step towards Root–Microbe Rhizosphere Interactions, International Journal of Molecular Sciences, 2021, Vol. 22, No. 13, pp. 6655.Gunina A., Kuzyakov Y. Sugars in soil and sweets for microorganisms: Review of origin, content, composition and fate // Soil Biology and Biochemistry. 2015. Vol. 90. P. 87‒100.Gunina A., Kuzyakov Y., Sugars in soil and sweets for microorganisms: Review of origin, content, composition and fate, Soil Biology and Biochemistry, 2015, Vol. 90, pp. 87‒100.IUSS Working Group WRB, World Reference Base for Soil resources 2014, International Soil Classification System for Naming Soils and Creating Legends for Soil Maps. World Soil Resources Reports No. 106 (FAO, Rome, 2014).IUSS Working Group WRB, World Reference Base for Soil resources 2014, International Soil Classification System for Naming Soils and Creating Legends for Soil Maps. World Soil Resources Reports No. 106 (FAO, Rome, 2014).Junya L., Xiaoliang Y., Le G., Qian L., Zhiguo L., Li W., Yi L. Rhizosphere effects promote soil aggregate stability and associated organic carbon sequestration in rocky areas of desertification // Agriculture, Ecosystems & Environment. 2020. Vol. 304. P. 107‒126.Junya L., Xiaoliang Y., Le G., Qian L., Zhiguo L., Li W., Yi L., Rhizosphere effects promote soil aggregate stability and associated organic carbon sequestration in rocky areas of desertification, Agriculture, Ecosystems & Environment, 2020, Vol. 304, pp. 107‒126.Katayama A., Bhula R., Burns G. R., Carazo E., Felsot A., Hamilton D., Harris C., Kim Y.-H., Kleter G., Koedel W., Linders J., Peijnenburg J.G.M.W., Sabljic A., Stephenson R.G., Racke D.K., Rubin B., Tanaka K., Unsworth J., Wauchope R.D. Bioavailability of Xenobiotics in the Soil Environment // Reviews of Environmental Contamination and Toxicology / Whitacre D.M. (Ed.). New York: Springer New York, 2010. P. 1‒86.Katayama A., Bhula R., Burns G. R., Carazo E., Felsot A., Hamilton D., Harris C., Kim Y.-H., Kleter G., Koedel W., Linders J., Peijnenburg J.G.M.W., Sabljic A., Stephenson R.G., Racke D.K., Rubin B., Tanaka K., Unsworth J., Wauchope R.D., Bioavailability of Xenobiotics in the Soil Environment, In: Reviews of Environmental Contamination and Toxicology, Whitacre D.M. (Ed.), New York: Springer New York, 2010, pp. 1‒86.Kuzyakov Y. Review: Factors affecting rhizosphere priming effects // Journal of Plant Nutrition and Soil Science. 2002. Vol. 165. No. 4. P. 382‒396.Kuzyakov Y., Review: Factors affecting rhizosphere priming effects, Journal of Plant Nutrition and Soil Science, 2002, Vol. 165, No. 4, pp. 382‒ 396.Kuzyakov Y., Razavi B.S. Rhizosphere size and shape: Temporal dynamics and spatial stationarity // Soil Biology and Biochemistry. 2019. Vol. 135. P. 343‒360.Kuzyakov Y., Razavi B.S., Rhizosphere size and shape: Temporal dynamics and spatial stationarity, Soil Biology and Biochemistry, 2019, Vol. 135, pp. 343‒360.Misra B.B. Data normalization strategies in metabolomics: Current challenges, approaches, and tools // European Journal of Mass Spectrometry. 2020. Vol. 26. No. 3. P. 165‒174.Misra B.B., Data normalization strategies in metabolomics: Current challenges, approaches, and tools, European Journal of Mass Spectrometry, 2020, Vol. 26, No. 3, pp. 165‒174.Sangster J. LOGKOW Databank. Montreal: Sangster Res. Lab, 1994. 184 p.Sangster J., LOGKOW Databank, Montreal: Sangster Res. Lab, 1994, 184 p.Sokolova T.A. Specificity of soil properties in the rhizosphere: Analysis of literature data // Eurasian Soil Science. 2015. Vol. 48. No. 9. P. 968‒980.Sokolova T.A., Specificity of soil properties in the rhizosphere: Analysis of literature data, Eurasian Soil Science, 2015, Vol. 48, No. 9, pp. 968‒980.Sun H., Jiang S., Jiang C., Wu C., Gao M., Wang Q. A review of root exudates and rhizosphere microbiome for crop production // Environmental Science and Pollution Research. 2021. Vol. 28. No. 39. P. 54497‒54510.Sun H., Jiang S., Jiang C., Wu C., Gao M., Wang Q., A review of root exudates and rhizosphere microbiome for crop production, Environmental Science and Pollution Research, 2021, Vol. 28, No. 39, pp. 54497‒54510.Swenson T.L., Jenkins S., Bowen B.P., Northen T.R. Untargeted soil metabolomics methods for analysis of extractable organic matter // Soil Biology & Biochemistry. 2015. Vol. 80. P. 189‒198.Swenson T.L., Jenkins S., Bowen B.P., Northen T.R., Untargeted soil metabolomics methods for analysis of extractable organic matter, Soil Biology & Biochemistry, 2015, Vol. 80, pp. 189‒198.Team R.C. R: A language and environment for statistical computing // MSOR connections. 2014. Vol. 1.Team R.C., R: A language and environment for statistical computing, MSOR connections, 2014, Vol. 1.Vives-Peris V., de Ollas C., Gómez-Cadenas A., Pérez-Clemente R.M. Root exudates: from plant to rhizosphere and beyond // Plant Cell Reports. 2020. Vol. 39. No. 1. P. 3‒17.Vives-Peris V., de Ollas C., Gómez-Cadenas A., Pérez-Clemente R.M., Root exudates: from plant to rhizosphere and beyond, Plant Cell Reports, 2020, Vol. 39, No. 1, pp. 3‒17.Vranova V., Rejsek K., Skene K.R., Janous D., Formanek P. Methods of collection of plant root exudates in relation to plant metabolism and purpose: A review // Journal of Plant Nutrition and Soil Science. 2013. Vol. 176. No. 2. P. 175‒199.Vranova V., Rejsek K., Skene K.R., Janous D., Formanek P., Methods of collection of plant root exudates in relation to plant metabolism and purpose: A review, Journal of Plant Nutrition and Soil Science, 2013, Vol. 176, No. 2, pp. 175‒199.Wang C., Kuzyakov Y. Rhizosphere engineering for soil carbon sequestration // Trends in Plant Science. 2024. Vol. 29. No. 4. P. 447‒468.Wang C., Kuzyakov Y., Rhizosphere engineering for soil carbon sequestration, Trends in Plant Science, 2024, Vol. 29, No. 4, pp. 447‒468.Wang H., Ding Y., Zhang Y., Wang J., Freedman Z. B., Liu P., Cong W., Wang J., Zang R., Liu S. Evenness of soil organic carbon chemical components changes with tree species richness, composition and functional diversity across forests in China // Global Change Biology. 2023. Vol. 29. No. 10. P. 2852‒2864.Wang H., Ding Y., Zhang Y., Wang J., Freedman Z. B., Liu P., Cong W., Wang J., Zang R., Liu S., Evenness of soil organic carbon chemical components changes with tree species richness, composition and functional diversity across forests in China, Global Change Biology, 2023, Vol. 29, No. 10, pp. 2852‒2864.Yang S., Jansen B., Absalah S., Kalbitz K., Cammeraat E.L.H. Selective stabilization of soil fatty acids related to their carbon chain length and presence of double bonds in the Peruvian Andes // Geoderma. 2020. Vol. 373. P. 114414.Yang S., Jansen B., Absalah S., Kalbitz K., Cammeraat E.L.H., Selective stabilization of soil fatty acids related to their carbon chain length and presence of double bonds in the Peruvian Andes, Geoderma, 2020, Vol. 373, pp. 114414.Zhang Z., Wang W., Qi J., Zhang H., Tao F., Zhang R. Priming effects of soil organic matter decomposition with addition of different carbon substrates // Journal of Soils and Sediments. 2019. Vol. 19. No. 3. P. 1171‒1178.Zhang Z., Wang W., Qi J., Zhang H., Tao F., Zhang R., Priming effects of soil organic matter decomposition with addition of different carbon substrates, Journal of Soils and Sediments, 2019, Vol. 19, No. 3, pp. 1171‒1178.Zhao X., Tian P., Sun Z., Liu S., Wang Q., Zeng Z. Rhizosphere effects on soil organic carbon processes in terrestrial ecosystems: A meta-analysis // Geoderma. 2022. Vol. 412. P. 115739.Zhao X., Tian P., Sun Z., Liu S., Wang Q., Zeng Z., Rhizosphere effects on soil organic carbon processes in terrestrial ecosystems: A meta-analysis, Geoderma, 2022, Vol. 412, pp. 115739.

The authors declare that there are no conflicts of interest present.